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KMID : 0381920070370040239
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Korean Journal of Microscopy
2007 Volume.37 No. 4 p.239 ~ p.248
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Response of Metastatic Cancer Cells to Thermal Changes in vitro
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Ahn San-gil
Kwon Young-Ee
Choi Ho-Soon
Kwon Jung-Kyun
Yoo Jin-Young
Kim Jong-Ryong
Kim Won-Kyu
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Abstract
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Alteration of temperature is one of cancer therapies. In general, severe hyperthermia (around 43??C) and hypothermia (around 18??C) trigger apoptosis through mitochondria, though the specific mechanism is still unknown. CC-t6 and GB-d1 cell lines, which were originally derived from human cholangiocarcinoma and gall bladder cancer, were established from a metastatic lymph node. To investigate the mechanism of metastatic cancer cell response to thermal stresses, hyperthermia (37??C → 43??C) and hypothermia (37??C → 17.4??C) were designed. Thermal stresses did not induce apoptosis but necrotic cell death. Any alterations of caspase-3, -9, cytochrome c, Bax, and Bcl-2 were not found in both hyperthermia and hypothermia exposed cells using western blot analysis. In the transmission electron microscopy, typical necrotic, but not apoptotic, changes were observed. These results suggest that temperature changes induce cell death through necrotic pathway in metastatic cancer in vitro, and it can be one of effective anticancer methods.
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KEYWORD
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Thermal stress, Necrosis, Apoptosis, Anti-cancer immunity, Metastatic cancer
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